A note about automated compensation wizards – sometimes they don’t match up the correct controls, so make sure the PE single stain control is assigned to PE, the APC single stain control is assigned to APC, etc. It’s likely that you didn’t set up the gates in your automated compensation wizard correctly (see more on that here), or you tried to do manual compensation and made a mistake. The easiest explanation for this situation is that you just didn’t calculate compensation correctly, so you need to go back and fix it. Step 3a: Solutions for errors in both controls and full stains
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